The Total Flavonoid Assay is a laboratory technique used to quantify the total flavonoid content in various samples such as plant extracts, foods, and biological fluids. Flavonoids, known for their antioxidant properties and health benefits, are a group of natural substances with variable phenolic structures. This assay typically involves the reaction of flavonoids in the sample with a colorimetric reagent, like aluminium chloride, which results in a colour change that is proportional to the flavonoid concentration. The intensity of this colour change is measured using a spectrophotometer. This method is essential in fields like nutrition, pharmacology, and botanical studies, as it helps in evaluating the health-promoting potential of foods and plants due to their flavonoid composition.
Kit components
Dilution Buffer
Reagent A
Standard
Reagent B
Reagent C
Reagent D