ABTS Assay

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The ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assay is a widely used method for assessing the antioxidant capacity of a range of substances, including plant extracts, food items, and biological fluids. This versatile assay works by generating a blue-green ABTS radical cation (ABTS•+) that absorbs light at 734 nm. Antioxidants present in the test sample reduce this radical cation, leading to a decrease in absorbance, a change that is directly proportional to the antioxidant capacity of the sample. This method is highly valued for its simplicity, sensitivity, and broad applicability in fields such as food science, pharmaceuticals, and academic research, where it's used to study oxidative stress, aging, and the benefits of natural products.

Kit components

Reagent A

Reagent B

Standard

Dilution buffer A

Dilution buffer B